Cytokine modulators and related methods of use

ABSTRACT

A composition for modulating cytokines to regulate an inflammatory or immunomodulatory response. The composition can include at least one of rosehips, blueberry, blackberry, elderberry, cranberry, rosemary, clove, feverfew, nettle root, artichoke, reishi mushroom, olive extract, green tea extract, grape seed extract, resveratrol, viniferin,  Aframomum melegueta, boswellia serrata  extract,  boswellia forte , ipriflavone, tocotrienols, evening primrose oil, INM-176, borage oil, krill oil, at least one type of xanthophyll (e.g., astaxanthin), green coffee extract and ferulic acid. Specifically, a composition of the invention can include: rosehips and at least one of blackberry, blueberry, elderberry, and optionally krill oil; or rosehips, resveratrol and at least one of  Aframomum melegueta  and astaxanthin. Based on the cytokine modulation and cytokine response inhibition of the composition, it can be used to regulate an immunomodulatory and/or inflammatory response, and subsequently treat diseases and/or abnormal conditions associated with inflammatory response, for example, cardiovascular conditions, arthritis, osteoporosis and Alzheimer&#39;s disease.

This application is a continuation-in-part of U.S. patent application Ser. No. 11/200,412, filed Aug. 8, 2005, which is a continuation-in-part of U.S. patent application Ser. No. 10/938,093, filed Sep. 10, 2004 and PCT Application Serial No. PCT/IB2004/051742, filed Sep. 10, 2004, and claims the benefit of U.S. Provisional Application Ser. No. 60/502,755, filed Sep. 12, 2003 and U.S. Provisional Application Ser. No. 60/710,730, filed Aug. 23, 2005, all of which are hereby incorporated in their entirety by reference.

BACKGROUND

The present invention relates to inflammation within the body, and more particularly, to regulating inflammation to treat conditions and diseases associated therewith.

Inflammation has been linked to a variety of conditions and diseases that affect the body. For example, inflammation within joints is known to worsen the symptoms of and structural deformities caused by arthritis and rheumatoid diseases, such as bursitis, tendonitis, myositis and osteoarthritis, as well as bone and joint destructive diseases, such as osteoporosis.

Inflammation also is known to contribute to a variety of cardiovascular and metabolic disease processes, such as atherosclerosis, thrombosis, and insulin resistance associated with obesity. Atherosclerosis may increase the chances of stroke and myocardial infarction and insulin resistance may lead to diabetes.

Inflammation is also thought to contribute to the development of neurological disorders, for example, Alzheimer's disease.

Indeed a large body of research now links inflammation with a wide variety of chronic degenerative diseases. This research has identified certain cells—macrophages—that produce pro-inflammatory chemicals—cytokines—which induce signaling cascades that provide an inflammatory response. These cytokines play a role in inflammatory reaction in response to foreign and infectious agents, traumatic or chronic injury, and abnormal chemical or physical stresses.

Accordingly, treatments have been developed to regulate the release of inflammatory cytokines, or the signaling of inflammatory cytokines, specifically the interleukin-1 (IL-1) cytokine from macrophages. For example, U.S. Pat. No. 5,635,478 to Vignery discloses the use of calcitonin gene related peptide (CGRP) to regulate IL-1 release, and thereby treat rheumatoid arthritis. Although highly specific CGRP is effective at regulating IL-1, its use is cost prohibitive and presently it is undetermined whether this compound has a toxic effect with prolonged use.

BRIEF SUMMARY

The aforementioned problems are overcome in the present invention which provides a composition that regulates interleukin cytokines and/or regulates a physiological response caused by interleukin cytokines. This regulation is effective in controlling an immune response and/or an inflammatory condition. In one aspect, the composition can comprise rosehips and at least one of blackberry, blueberry and elderberry. In further aspect, the composition can comprise rosehips, resveratrol, and at least one of blackberry, blueberry, elderberry, and cranberry. In a further aspect, the composition can comprise rosehips, at least one of resveratrol and viniferin, and at least one of blackberry, blueberry, elderberry, and cranberry. In another aspect, the composition can comprise rosehips and krill oil. In yet another aspect, the composition can comprise rosehips, blackberry, blueberry, elderberry and krill oil. In a further aspect, the composition can comprise rosehips, resveratrol and Aframomum melegueta. In another aspect, the composition can comprise rosehips, at least one of resveratrol and viniferin, and Aframomum melegueta. In an even further aspect, the composition can comprise rosehips, resveratrol and astaxanthin. In another aspect, the composition can comprise rosehips, Aframomum melegueta, and at least one of blackberry, blueberry, elderberry, and cranberry.

In a further aspect, the composition can comprise at least one ingredient chosen from rosehips, blueberry, blackberry, elderberry, cranberry, rosemary, clove, feverfew, nettle root, artichoke, reishi mushroom, olive extract, green tea extract (epigallocatechin gallate), grape seed extract, resveratrol, viniferin, Aframomum melegueta, boswellia serrata extract, boswellia forte, ipriflavone, tocotrienols, evening primrose oil, INM-176, borage oil, krill oil, at least one type of xanthophyll (e.g., astaxanthin), green coffee extract (chlorogenic acid), and ferulic acid. In a more specific aspect, the composition can comprise rosehips, nettle root, olive extract and artichoke. In yet another specific aspect, the composition can comprise rosehips, resveratrol and astaxanthin.

In another aspect, the invention can provide methods for modulating or controlling an immune response and/or an inflammatory condition in a subject, the method comprising administering to the subject an effective amount of the composition of the invention to modulate or control the immune response and/or the inflammatory condition. In a specific aspect, the composition can inhibit the function of an immunomodulatory or pro-inflammatory cell, for example, a macrophage and/or a leukocyte. In a more specific aspect, the composition can inhibit the expression of the genes that produce interleukin cytokines, for example, by preventing the genetic transcription of those genes. In an even more specific aspect, the composition can inhibit the interleukin cytokine inflammation response mechanism. In another specific aspect, the composition can modulate expression of an immunomodulatory or pro-inflammatory cell, for example by preventing an increase in expression, secretion, and/or synthesis levels of an immunomodulatory or pro-inflammatory cell. In these aspects, the composition can modulate or control pro-immunomodulatory and/or pro-inflammatory responses in skeletal mass, joints, muscle, tissue, arteries, veins, capillaries, and other organs, systems and/or cells.

In a further aspect, the invention can provide a method of regulating and/or controlling the function of immune cells, such as macrophages, leukocytes and lymphocytes, by administering an effective amount of the composition to a subject.

In another aspect, the invention can provide a method for regulating cytokine release, also referred to as secretion, from cells in a subject by administering to a subject a composition that modulates the synthesis, expression, or secretion of a mediator of inflammation.

In yet another aspect, the invention can provide a method that inhibits the response of cells to an interleukin cytokine by administering to a subject an effective amount of the composition of the invention. In a specific aspect, this administration can modulate the production of inflammation biomarkers, for example, C reactive protein, which is a biomarker produced by the liver that is indicative of excessive inflammation in the body.

In a further aspect, the invention can provide a method for treating a disease or abnormal condition caused by inflammation by administering a therapeutically effective amount of the composition. In a specific aspect, the disease or abnormal condition includes at least one of a cardiovascular disease or condition, thrombosis, a metabolic condition related to insulin resistance and obesity, a traumatic injury, arthritis, osteoporosis, and Alzheimer's disease. In a more specific aspect, the method can include administering the composition to a subject having an inflammatory condition to relieve or eliminate pain, tenderness, infection and/or discomfort following traumatic injuries, surgery or other events that may cause inflammation.

The present invention provides a composition and related methods for treating a variety of immunomodulatory- and inflammation-based conditions, symptoms and diseases. Because the ingredients used are readily available and relatively inexpensive, the present invention provides a simple and cost-effective solution for treating a variety of inflammation-caused ailments and conditions. Furthermore, because the ingredients are relatively stable, many can be mixed with other materials and provided in a multipurpose supplement or food product.

These and other objects, advantages and features of the invention will be more readily understood and appreciated by reference to the drawings and the detailed description of the invention.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is a sectional view of a blood vessel of a subject having atherosclerotic plaque development before being treated with the composition of the present invention in Example 3;

FIG. 2 is a second sectional view of the blood vessel and a liver of a subject that illustrates the effects of interleukin cytokines in atherosclerotic plaque development in Example 3; and

FIG. 3 is a third sectional view of the blood vessel and the liver of a subject that illustrates the effects of the composition on interleukin cytokines in atherosclerotic plaque development in Example 3.

FIG. 4 is a line graph illustrating Baseline vs. 12 Weeks Normalized CRP Expression Levels in the Total Study Population. In FIG. 4, “RH” indicates Rosehips, “B” indicates a berry ingredient selected from at least one of blackberry, blueberry, elderberry, and cranberry, “AM” indicates Aframomum melegueta, and “R” indicates resveratrol. The results reported in FIG. 4 are based on the daily administration of the following compositions for twelve consecutive weeks: 1200 mg rosehips, 165 mg blueberry powdered extract, 332 mg blackberry powdered extract, and 300 mg Aframomum melegueta extract; 1200 mg rosehips, 165 mg blueberry powdered extract, 332 mg blackberry powdered extract, and 40 mg of resveratrol; 1200 mg of rosehips, 40 mg of resveratrol, and 300 mg of Aframomum melegueta; or placebo.

FIG. 5 is a graph illustrating Baseline vs. 12 Weeks Normalized CRP Expression in the Total Study Population. In FIG. 5, “RH” indicates Rosehips, “B” indicates a berry ingredient selected from at least one of blackberry, blueberry, elderberry, and cranberry, “AM” indicates Aframomum melegueta, and “R” indicates resveratrol. The results reported in FIG. 5 are based on the daily administration of the following compositions for twelve consecutive weeks: the first bar is based on administration of 1200 mg rosehips, 165 mg blueberry powdered extract, 332 mg blackberry powdered extract, and 300 mg Aframomum melegueta extract; the second bar is based on administration of 1200 mg rosehips, 165 mg blueberry powdered extract, 332 mg blackberry powdered extract, and 40 mg of resveratrol; the third bar is based on administration of 1200 mg of rosehips, 40 mg of resveratrol, and 300 mg of Aframomum melegueta.

FIG. 6 is a graph illustrating Baseline vs. 12 Weeks Normalized CRP Expression Levels in Test Subjects having a Pattern 1 Genotype. In FIG. 6, “RH” indicates Rosehips, “B” indicates a berry ingredient selected from at least one of blackberry, blueberry, elderberry, and cranberry, “AM” indicates Aframomum melegueta, and “R” indicates resveratrol. The results reported in FIG. 6 are based on the daily administration of the following compositions for twelve consecutive weeks: 1200 mg rosehips, 165 mg blueberry powdered extract, 332 mg blackberry powdered extract, and 300 mg Aframomum melegueta extract; 1200 mg rosehips, 165 mg blueberry powdered extract, 332 mg blackberry powdered extract, and 40 mg of resveratrol; 1200 mg of rosehips, 40 mg of resveratrol, and 300 mg of Aframomum melegueta; or placebo.

FIG. 7 is a graph illustrating Baseline vs. 12 Weeks Normalized CRP Expression in Test Subjects Having a Pattern 1 Genotype. In FIG. 7, “RH” indicates Rosehips, “B” indicates a berry ingredient selected from at least one of blackberry, blueberry, elderberry, and cranberry, “AM” indicates Aframomum melegueta, and “R” indicates resveratrol. The results reported in FIG. 7 are based on the daily administration of the following compositions for twelve consecutive weeks: the first bar is based on administration of 1200 mg rosehips, 165 mg blueberry powdered extract, 332 mg blackberry powdered extract, and 300 mg Aframomum melegueta extract; the second bar is based on administration of 1200 mg rosehips, 165 mg blueberry powdered extract, 332 mg blackberry powdered extract, and 40 mg of resveratrol; the third bar is based on administration of 1200 mg of rosehips, 40 mg of resveratrol, and 300 mg of Aframomum melegueta.

FIG. 8 illustrates Baseline vs. 12 Weeks Normalized Ex vivo IL-1 Expression in the Total Study Population. In FIG. 8, “RH” indicates Rosehips, “B” indicates a berry ingredient selected from at least one of blackberry, blueberry, elderberry, and cranberry, “AM” indicates Aframomum melegueta, and “R” indicates resveratrol. The results reported in FIG. 8 are based on the daily administration of the following compositions for twelve consecutive weeks: the first bar is based on administration of 1200 mg rosehips, 165 mg blueberry powdered extract, 332 mg blackberry powdered extract, and 300 mg Aframomum melegueta extract; the second bar is based on administration of 1200 mg rosehips, 165 mg blueberry powdered extract, 332 mg blackberry powdered extract, and 40 mg of resveratrol; the third bar is based on administration of 1200 mg of rosehips, 40 mg of resveratrol, and 300 mg of Aframomum melegueta.

FIG. 9 illustrates Baseline vs. 12 Weeks Normalized Ex vivo IL-1 Expression in Human Subjects Having a Pattern 1 Genotype. In FIG. 9, “RH” indicates Rosehips, “B” indicates a berry ingredient selected from at least one of blackberry, blueberry, elderberry, and cranberry, “AM” indicates Aframomum melegueta, and “R” indicates resveratrol. The results reported in FIG. 9 are based on the daily administration of the following compositions for twelve consecutive weeks: 1200 mg rosehips, 165 mg blueberry powdered extract, 332 mg blackberry powdered extract, and 300 mg Aframomum melegueta extract; 1200 mg rosehips, 165 mg blueberry powdered extract, 332 mg blackberry powdered extract, and 40 mg of resveratrol; 1200 mg of rosehips, 40 mg of resveratrol, and 300 mg of Aframomum melegueta; or placebo.

FIG. 10 illustrates Baseline vs. 12 Weeks Normalized Ex vivo IL-1 Expression in Human Subjects Have Pattern 1 Genotype. In FIG. 10, “RH” indicates Rosehips, “B” indicates a berry ingredient selected from at least one of blackberry, blueberry, elderberry, and cranberry, “AM” indicates Aframomum melegueta, and “R” indicates resveratrol. The results reported in FIG. 10 are based on the daily administration of the following compositions for twelve consecutive weeks: the first bar is based on administration of 1200 mg rosehips, 165 mg blueberry powdered extract, 332 mg blackberry powdered extract, and 300 mg Aframomum melegueta extract; the second bar is based on administration of 1200 mg rosehips, 165 mg blueberry powdered extract, 332 mg blackberry powdered extract, and 40 mg of resveratrol; the third bar is based on administration of 1200 mg of rosehips, 40 mg of resveratrol, and 300 mg of Aframomum melegueta.

FIG. 11 illustrates Baseline vs. 12 Weeks Normalized IL-1 Gene Expression in the total study population. In FIG. 11, “RH” indicates Rosehips, “B” indicates a berry ingredient selected from at least one of blackberry, blueberry, elderberry, and cranberry, “AM” indicates Aframomum melegueta, and “R” indicates resveratrol. The results reported in FIG. 11 are based on the daily administration of the following compositions for twelve consecutive weeks: 1200 mg rosehips, 165 mg blueberry powdered extract, 332 mg blackberry powdered extract, and 300 mg Aframomum melegueta extract; 1200 mg rosehips, 165 mg blueberry powdered extract, 332 mg blackberry powdered extract, and 40 mg of resveratrol; 1200 mg of rosehips, 40 mg of resveratrol, and 300 mg of Aframomum melegueta; or placebo.

FIG. 12 illustrates Baseline vs. 12 Weeks Normalized IL-1 Gene Expression in the total study population. In FIG. 12, “RH” indicates Rosehips, “B” indicates a berry ingredient selected from at least one of blackberry, blueberry, elderberry, and cranberry, “AM” indicates Aframomum melegueta, and “R” indicates resveratrol. The results reported in FIG. 12 are based on the daily administration of the following compositions for twelve consecutive weeks: the first bar is based on administration of 1200 mg rosehips, 165 mg blueberry powdered extract, 332 mg blackberry powdered extract, and 300 mg Aframomum melegueta extract; the second bar is based on administration of 1200 mg rosehips, 165 mg blueberry powdered extract, 332 mg blackberry powdered extract, and 40 mg of resveratrol; the third bar is based on administration of 1200 mg of rosehips, 40 mg of resveratrol, and 300 mg of Aframomum melegueta.

FIG. 13 illustrates Baseline vs. 12 Weeks Normalized IL-1 Gene Expression in Test Subjects having a Pattern 1 Genotype. In FIG. 13, “RH” indicates Rosehips, “B” indicates a berry ingredient selected from at least one of blackberry, blueberry, elderberry, and cranberry, “AM” indicates Aframomum melegueta, and “R” indicates resveratrol. The results reported in FIG. 13 are based on the daily administration of the following compositions for twelve consecutive weeks: 1200 mg rosehips, 165 mg blueberry powdered extract, 332 mg blackberry powdered extract, and 300 mg Aframomum melegueta extract; 1200 mg rosehips, 165 mg blueberry powdered extract, 332 mg blackberry powdered extract, and 40 mg of resveratrol; 1200 mg of rosehips, 40 mg of resveratrol, and 300 mg of Aframomum melegueta; or placebo.

FIG. 14 illustrates Baseline vs. 12 Weeks Normalized IL-1 Gene Expression in Test Subjects having Pattern 1 Genotype. In FIG. 14, “RH” indicates Rosehips, “B” indicates a berry ingredient selected from at least one of blackberry, blueberry, elderberry, and cranberry, “AM” indicates Aframomum melegueta, and “R” indicates resveratrol. The results reported in FIG. 14 are based on the daily administration of the following compositions for twelve consecutive weeks: the first bar is based on administration of 1200 mg rosehips, 165 mg blueberry powdered extract, 332 mg blackberry powdered extract, and 300 mg Aframomum melegueta extract; the second bar is based on administration of 1200 mg rosehips, 165 mg blueberry powdered extract, 332 mg blackberry powdered extract, and 40 mg of resveratrol; the third bar is based on administration of 1200 mg of rosehips, 40 mg of resveratrol, and 300 mg of Aframomum melegueta. The results reported in FIG. 14 demonstrate that Composition 2 (second bar) significantly inhibits expression and/or synthesis of IL-1 in test subjects having a Pattern 1 genotype.

FIG. 15 illustrates Baseline vs. 12 Weeks Normalized II-1 Gene Expression in Test Subjects having a non-Pattern 1 Genotype. In FIG. 15, “RH” indicates Rosehips, “B” indicates a berry ingredient selected from at least one of blackberry, blueberry, elderberry, and cranberry, “AM” indicates Aframomum melegueta, and “R” indicates resveratrol. The results reported in FIG. 15 are based on the daily administration of the following compositions for twelve consecutive weeks: 1200 mg rosehips, 165 mg blueberry powdered extract, 332 mg blackberry powdered extract, and 300 mg Aframomum melegueta extract; 1200 mg rosehips, 165 mg blueberry powdered extract, 332 mg blackberry powdered extract, and 40 mg of resveratrol; 1200 mg of rosehips, 40 mg of resveratrol, and 300 mg of Aframomum melegueta; or placebo.

DETAILED DESCRIPTION I. The Composition

A composition of the invention can include one or more ingredients chosen from rosehips, blueberry, blackberry, elderberry, cranberry, rosemary, clove, feverfew, nettle root, artichoke, reishi mushroom, olive extract, green tea extract (epigallocatechin gallate), grape seed extract, resveratrol, viniferin, Aframomum melegueta, boswellia serrata extract, boswellia forte, ipriflavone, tocotrienols, evening primrose oil, INM-176, borage oil, krill oil, at least one type of xanthophyll (e.g., astaxanthin), green coffee extract (chlorogenic acid), and ferulic acid. Specifically, a composition of the invention can include rosehips and at least one of blackberry, blueberry, elderberry and krill oil. A composition also can include rosehips, resveratrol and Aframomum melegueta. Another composition can include rosehips, resveratrol and astaxanthin. Further, a composition of the present invention can include rosehips, Aframomum melegueta, and at least one of viniferin and resveratrol. A further composition of the invention can include rosehips, Aframomum melegueta, and at least one of blueberry, blackberry, elderberry, and cranberry. Another composition can include rosehips, resveratrol and at least one of blueberry, blackberry, elderberry, and cranberry. A further composition can include rosehips, at least one of viniferin and resvertrol, and at least one of blueberry, blackberry, elderberry, and cranberry. The composition can be administered in any of the dosages recited herein to inhibit cytokine expression, production, reception, secretion and/or release, as well as inhibit the cytokine response, thereby reducing or eliminating an immunomodulatory and/or inflammatory response.

Acceptable dosages of the ingredients that may be effective at modulating cytokines, for example, regulating the production, reception, secretion and/or release of exemplary cytokines, such as IL-1 and/or IL-6, are presented in Table I below. Each dosage in Table I is an estimated effective daily dosage in milligrams. Furthermore, all the dosages in Table I are presented in ranges of from about the recited lower limit to about the upper limit. For example, the nettle Dosage A recites “250-2500”, which represents a dosage of about 250 to about 2500 milligrams of nettle per day.

TABLE I Acceptable Dosages for Ingredients to Modulate Cytokines Ingredient Dosage A Dosage B Nettle extract 250-2500  500-1250 Artichoke 150-1500 300-750 Feverfew 50-500 100-250 Reishi mushroom 300-3000  600-1500 Olive extract 300-3000  600-1500 Green tea extract 150-1500 300-750 Grape seed extract 100-1000 200-500 Aframomum melegueta extract 150-1500 300-750 Boswellia serrata extract 350-3500  700-1750 Ipriflavone 100-1000 200-500 Tocotrienols 50-500 100-250 Evening primrose oil 500-5000 1000-2500 INM-176 100-1000 200-500 Borage Oil 500-5000 1000-2500 Krill Oil 300-3000  600-1500 Green coffee extract (chlorgenic 100-1000 200-500 acid) Ferulic acid 100-1000 200-500 Rosehips 50-500  500-5000 Blackberry powder 100-1000 200-500 Blueberry powder 200-2000 300-1500 Cranberry extract 100-1000 200-500 Rosemary extract 100-1000 200-500 Clove extract 100-1000 200-500 Resveratrol 100-1000 200-500 Viniferin 100-1000 200-500 Elderberry extract 400-4000  700-2500

The ingredients identified above in Table I are readily commercially available. Depending on the application and/or the supplier, the ingredient may be an extract of a specific potency, a pure ingredient, an ingredient mixed with excipients, and in a variety of physical forms, e.g., liquid or powder. The ingredient identified as INM-176 is a compound of unknown composition available from Scigenic Company, Ltd. of Seoul, Korea.

More particularly, the composition can include one or more than one rosehip ingredient. Examples of rosehip ingredients include, without limitation, dried rosehips, rosehip oil, and rosehip extracts. A rosehip ingredient can be obtained from any of the multiple species of plants that belong to the Rosa family, for example Rosa canina. Moreover, rosehips can include the fruit, petals and/or seeds of the Rosa plants.

Any method can be used to prepare a rosehips ingredient. As an example, conventional harvesting and drying methods can be used to prepare dried rosehips. Rosehip oil can be produced with standard methods and processed with cellulose for tableting or powdered compositions. In addition, rosehips can be obtained commercially from MB North America of Torrance, Calif.

A composition of the invention can contain one or more than one rosehips ingredient. For example, a dietary supplement can contain dried rosehips as well as rosehips extract. In addition, a composition can contain any amount of a rosehips component. For example, at least about 1 percent (e.g., at least about 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 50, 60, 70, 80, or 90 percent) of a dietary supplement can be a rosehips ingredient. Typically, a composition contains between about 500 mg and about 5000 mg of a rosehips ingredient and acceptably between about 500 mg and about 1500 mg.

Even more particularly, the composition can include one or more than one blackberry ingredient. Examples of blackberry ingredients include, without limitation, dried blackberry, blackberry powder, and blackberry extracts. A blackberry ingredient can be obtained from any of the multiple species of plants that belong to the Rubus family, for example Rubus fruticosus.

Any method can be used to prepare a blackberry ingredient. As an example, conventional harvesting, drying and powdering methods can be used to prepare blackberry powder. In addition, blackberry can be obtained commercially from Van Drunen Farms Futureceuticals of Santa Rosa, Calif.

A composition of the invention can contain one or more than one blackberry ingredient. For example, a dietary supplement can contain blackberry powder as well as blackberry extract. In addition, a composition can contain any amount of a blackberry component. For example, at least about 1 percent (e.g., at least about 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 50, 60, 70, 80, or 90 percent) of a dietary supplement can be a blackberry ingredient. Typically, a composition contains between about 100 mg and about 1000 mg of a blackberry ingredient.

Further particularly, the composition can include one or more than one blueberry ingredient. Examples of blueberry ingredients include, without limitation, dried blueberry, blueberry powder, and blueberry extracts. A blueberry ingredient can be obtained from any of the multiple species of plants that belong to the Vaccinium family, for example, Vaccinium corymbosum.

Any method can be used to prepare a blueberry ingredient. As an example, conventional harvesting, drying and powdering methods can be used to prepare blueberry powder. In addition, blueberry can be obtained commercially from Van Drunen Farms Futureceuticals of Santa Rosa, Calif.

A composition of the invention can contain one or more than one blueberry ingredient. For example, a dietary supplement can contain blueberry powder as well as blueberry extract. In addition, a composition can contain any amount of a blueberry component. For example, at least about 1 percent (e.g., at least about 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 50, 60, 70, 80, or 90 percent) of a dietary supplement can be a blueberry ingredient. Typically, a composition contains between about 200 mg and about 2000 mg of a blueberry ingredient.

Even more particularly, the composition can include one or more than one elderberry ingredient. Examples of elderberry ingredients include, without limitation, elderberry extracts, dried elderberry and elderberry powder. An elderberry ingredient can be obtained from any of the multiple species of plants that belong to the Sambucus family, for example, Sambucus canadensis.

Any method can be used to prepare an elderberry ingredient. As an example, conventional extraction techniques can be used to prepare an elderberry extract. In addition, elderberry can be obtained commercially from Artemis International of Fort Wayne, Ind.

A composition of the invention can contain one or more than one elderberry ingredient. For example, a dietary supplement can contain elderberry powder as well as elderberry extract. In addition, a composition can contain any amount of an elderberry component. For example, at least about 1 percent (e.g., at least about 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 50, 60, 70, 80, or 90 percent) of a dietary supplement can be an elderberry ingredient. Typically, a composition contains between about 400 mg and about 4000 mg of an elderberry ingredient.

Furthermore, the composition can include Aframomum melegueta. Examples of Aframomum melegueta ingredients include, without limitation, pulverized Aframomum melegueta seeds, Aframomum melegueta powder, and Aframomum melegueta extracts.

Any method can be used to prepare an Aframomum melegueta ingredient. As an example, conventional harvesting, drying and extracting methods can be used to prepare Aframomum melegueta extracts. In addition, Aframomum melegueta can be obtained commercially from Frontier Natural Products Cooperative of Norway, Iowa.

A composition of the invention can contain one or more than one Aframomum melegueta ingredient. For example, a dietary supplement can contain Aframomum melegueta powder as well as Aframomum melegueta extract. In addition, a composition can contain any amount of a Aframomum melegueta component. For example, at least about 1 percent (e.g., at least about 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 50, 60, 70, 80, or 90 percent) of a dietary supplement can be a Aframomum melegueta ingredient. Typically, a composition contains between about 500 mg and about 1500 mg of a Aframomum melegueta ingredient.

A composition of the invention can include krill oil. Krill oil can be obtained from any member of the Euphausia family, for example Euphausia superba. Conventional oil producing techniques can be used to obtain the krill oil. In addition, krill oil can be obtained commercially from Neptune Technologies and Bioresources of Quebec, Canada.

A composition can contain any amount of krill oil. For example, at least about 1 percent (e.g., at least about 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 50, 60, 70, 80, or 90 percent) of a dietary supplement can be a krill oil. Typically, a composition contains between about 300 mg and about 3000 mg of a krill oil ingredient.

Where the composition includes resveratrol, the resveratrol can be obtained from an extract of grape skin or other grape components, including the grape vine. Resveratrol can be present in the composition in one or more different forms, for example, extract form and powder form. Resveratrol can be obtained commercially from Charles Bowman & Co. of Holland, Mich.

The composition can further include a resveratrol ingredient that comprises resveratrol and/or derivatives thereof. For example, a resveratrol ingredient may be any oligomer of resveratrol, such as ε-viniferin, which is a dimer of resveratrol. In a further example, the resveratrol ingredient can include resveratrol in combination with derivatives of resveratrol. In one example, the resvertrol ingredient is Resveravine® (BioSerae Laboratoires, S.A., Parc Technologique du Lauragais, Bram, France), which contains both resveratrol and ε-viniferin.

A composition can contain any amount of a resveratrol ingredient. For example, at least about 1 percent (e.g., at least about 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 50, 60, 70, 80, or 90 percent) of a dietary supplement can be a resveratrol ingredient. Typically, a composition contains between about 100 mg and about 1000 mg of a resveratrol ingredient.

Where the composition includes at least one type of xanthophyll, the xanthophyll can be astaxanthin. Astaxanthin can be obtained from natural sources or synthesized. For example, astaxanthin can be obtained from algae, fungi and/or crustaceans. One type of astaxanthin can be obtained commercially from Cyanotech Corporation of Kailua-Kona, Hi.

A composition can contain any amount of an astaxanthin ingredient. For example, at least about 1 percent (e.g., at least about 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 50, 60, 70, 80, or 90 percent) of a dietary supplement can be astaxanthin. Typically, a composition contains between about 0.5 mg and about 50 mg of an astaxanthin ingredient.

The composition can include one or more pharmaceutically acceptable excipients, for example, croscarmellose sodium, maltodextrin, silicified microcrystalline cellulose, silicon dioxide, stearic acid, hydroxylpropyl methyl cellulose (HPMC), lactose, glucose, sucrose, corn starch, potato starch, cellulose acetate, ethyl cellulose and the like. Diluents and other additives such as one or more pharmaceutically acceptable binding agents, fillers, supports, thickening agents, taste-improving agents, coloring agents, preservatives, proteins, anti-microbials, chelating agents, inert gases, stabilizers, regulators, emulsifiers or mixtures thereof, can be used depending on the form of the composition employed.

The ingredients of the composition can be processed into forms having varying delivery systems. For example, the ingredients can be processed and included in capsules, tablets, gel tabs, lozenges, strips, granules, powders, concentrates, solutions, lotions, creams or suspensions. The ingredients can also be administered into the respiratory tract, e.g. in the treatment of asthma, anaphylactic or and other acute shock conditions via a spray, mist or aerosol. The ingredients can be formulated, individually or in combination, with other foods to provide pre-measured nutritional supplements, supplemented foods, for example, single serving bars. In one example, the composition can include one ingredient administered in tablet form, and another ingredient administered in capsule form. More generally, the composition can be formulated for a variety of administration routes, for example, intravenously, intramuscularly, subcutaneously, nasally, sublingually, intrathecally, topically or intradermally.

The dosages of the composition may be administered in increments determined by the disease or condition being treated. For example, in the treatment of arthritis, the composition may be administered in multiple successive dosages, spaced as frequently as about 6 to about 12 hours apart, and as long as about 2 weeks apart. Daily dosages may be administered for a period to obtain symptomatic relief from inflammatory tenderness, swelling and pain. The dosages may be adjusted to maintain the relief from these symptoms. Treatment may be a period of weeks or months, or optionally, indefinitely for chronic diseases or conditions.

II. Method of Manufacturing the Composition

An exemplary composition of the invention including rosehips, blackberry powder, blueberry powder and elderberry extract can be manufactured in tablet form according to the following processes. Other administration mediums, such as gel tabs, capsules, liquid and sustained release formulations, etc. can be formulated and prepared according to manufacturing techniques well known in the pharmaceutical industry.

Rosehips, blackberry powder, and blueberry powder in the amounts recited in Dosage A in Table II above are added to silicone dioxide and placed in a polybag and blended until uniformly and homogeneously mixed. This resultant blend is placed in a Patterson Kelley (P.K.) 100 blender available from Patterson-Kelley Co., East Stroudsburg, Pa. The following ingredients in the order listed are then passed through a Sweco separator equipped with a 20-mesh screen available from Sweco, Inc. of Florence, Ky. directly into the P.K. 100 blender: dextrose, croscarmellose sodium, and silicified microcrystalline cellulose. The resultant composition is blended in the P.K. 100 blender for 15 minutes.

A vegetable-based stearic acid powder is then passed through the Sweco separator directly into the P.K. 100 blender. The resultant mixture is blended for an additional five minutes. The resulting mixture is discharged into totes or super sacks, compressed, and punched into tablets by conventional apparatus. An acceptable range weight for each individual tablet is from about 250 mg to about 500, mg. The amount of rosehips in each tablet is about 1200 mg, the amount of blackberry is about 165 mg and the amount of blueberry is about 330 mg. Tablets manufactured according to this process can be used in the in vivo testing described herein.

A soft gel capsule of the composition can be manufactured to include krill oil. This capsule can be manufactured using conventional capsule manufacturing techniques. The amount of krill oil in each capsule is about 300 mg. These capsules can be administered alone or in conjunction with the tablets of other ingredients of the composition as part of the in vivo testing described herein.

Other compositions of the invention including either rosehips and blackberry powder, or rosehips and blueberry powder, can be manufactured in tablet form according to the tableting process described above. The amount of rosehips in the rosehips/blackberry tablet is about 300 mg, and the amount of blackberry is about 80 mg. The amount of rosehips in the rosehips/blueberry tablet is about 300 mg, and the amount of blueberry is about 40 mg. Tablets manufactured according to this process can be used in the in vivo testing described herein as desired.

Another composition including rosehips, resveratrol and Aframomum melegueta can be manufactured in table form according to the tableting process described above, with about 300 mg rosehips, 10 mg resveratrol and 75 mg Aframomum melegueta extract.

Another composition of the invention including rosehips, resveratrol and astaxanthin can be manufactured in table form according to the tableting process described above, with about 300 mg rosehips, 10 mg resveratrol and 1 mg astaxanthin.

Yet another composition of the invention including olive extract, artichoke, nettle root and rosehips can be manufactured in tablet form according to the following process. Olive extract, artichoke, nettle root and rosehips in the amounts recited in Dosage A in Table II above are added to silicone dioxide and placed in a polybag and blended until uniformly and homogeneously mixed. This resultant blend is placed in a Patterson Kelley (P.K.) 100 blender available from Patterson-Kelley Co., East Stroudsburg, Pa. The following ingredients in the order listed are then passed through a Sweco separator equipped with a 20-mesh screen available from Sweco, Inc. of Florence, Ky., directly into the P.K. 100 blender: dextrose, croscarmellose sodium, and silicified microcrystalline cellulose. The resultant composition is blended in the P.K. 100 blender for 15 minutes.

A vegetable-based stearic acid powder is then passed through the Sweco separator directly into the P.K. 100 blender. The resultant mixture is blended for an additional five minutes. The resulting mixture is discharged into totes or super sacks, compressed, and punched into tablets by conventional apparatus. An acceptable range weight for each individual tablet is from about 250 mg to about 500 mg.

III. Selection of Ingredients for the Composition

The present invention can include an ingredient screening process for selecting ingredients that are optimal for particular health conditions, and that can be used in the composition as desired. For example, in the case of a nutritional supplement for modulating IL-1, the active ingredients can be selected via a unique screening process. The various factors include biological, commercial, regulatory, and descriptive discriminators. Some preferred discriminators and their weighted values are outlined Table 2. Preferably, the discriminators are assigned a weight based upon business or consumer driven objectives. In Table 2, a weight of 3 means that the discriminator carries heavy weighting or importance in the selection process (“Factor 3 Discriminator”); 2 means that the discriminator carries significant weight, but less than a weight factor of 3 in the selection process (“Factor 2 Discriminator”); and 1 carries the least weight as a discriminator (“Factor 1 Discriminator”).

TABLE II Discriminators for Selecting Cytokine Modulating Ingredients Discriminator Weight Performance in IL-1 screening assay 3 (EC <50 ug/ml) Supply Availability 3 Cost per kilogram of material 3 Raw Material Name 1 Botanical Identity/Parent Extract 1 Optional and secondary material and 2 suppliers Potential for exclusivity 2 Vertical integration possibility 2 Aqueous versus hydrophobic 2 compatibility Known active ingredients/Mechanism 2 of action Fractional bioavailability* 2 Efficacious daily dose based upon in 2 vitro EC 50 mg Efficacious daily dose based upon 2 literature (mg) Estimated raw material cost based 2 upon bioassay EC 50 and bioavailability (cost per month) Estimated raw material cost based 2 upon literature efficacy (cost per month) Safety/Toxicity status 3 Technical/Regulatory acceptance in 3 market country Representation in the source 2 company's products and in the general marketplace Intellectual property risks 2 *Fractional bioavailability is the percentage of the active compounds in an orally ingested ingredient (expressed as a fraction) that is absorbed into the body, and is generally available to body tissues via the circulation. For example, a fractional availability of 0.10 indicates that 10% of an orally ingested ingredient's actives are absorbed from the intestinal tract and are (for some period of time) transported in the circulation.

In the above example, the primary objective is to find an effective and commercially successful IL-1 modulator for human use. Selection of the best candidates is accomplished by summing the discriminator weights for each candidate and comparing total weighted score. Thus, performance of an active ingredient in modulating IL-1 is relevant, as is the expense to obtain the particular ingredient and the availability of the ingredient. Other factors relevant to commercial success are the ingredient's toxicity and safety as well as the technical and regulatory acceptance of the ingredient in the market where the composition will be sold. There may be cases where some ingredients do not meet one Factor 3 Discriminator, and the one Factor 3 Discriminator is different for each ingredient. In these cases, Factor 3 Discriminators are assigned weights and Factor 2 and Factor 1 Discriminators are also considered in the selection process.

In addition to the above ingredient screening process, the therapeutic composition or ingredient is preferably subjected to an ex vivo clinical evaluation to confirm the biological properties and effects. In particular, the subject consumes the therapeutic composition or ingredient. Serial blood samples are obtained from the subject before and following consumption over a time period when the ingredient's active components are likely to be absorbed by and transported within the circulatory system of the body, which is about 6 hours for most chemicals. These blood samples are added to an in vitro bioassay system which has an assay or test readout that is sensitive to changes in the physiology of the system of interest. The results of this test, referred to as an ex vivo test, are compared to the results from the in vitro clinical. The closer the results of the in vitro and ex vivo evaluations the greater the likelihood that the therapeutic will have desired effect within the body.

IV. Cytokine Inhibitory Potential Evaluation

The ingredients of the composition can modulate cytokines via at least two different biological mechanisms. In a first mechanism, the ingredients can inhibit the production, i.e., synthesis, of interleukin cytokines. In this mechanism, it is believed that the ingredients prevent the mRNA transcription of the genes that code for the production of the interleukin cytokines. Therefore, with genetic transcription blocked, the cytokines can not be synthesized by or in the body. In a second mechanism, the ingredients can inhibit the cascading response of chemicals and cells in the body to the interleukin cytokines. More particularly, the ingredients can prevent the production of—among other compounds—acute phase proteins, for example, C reactive protein (CRP). CRP is synthesized in the human liver by hepatocytes in response to interleukin cytokines, e.g., IL-1 and IL-6, under traumatic and/or inflammatory conditions. The ingredients can affect the response mechanism of cells, for example, liver cells, to interleukin cytokines so that those cells reduce or stop production of CRP.

In the following examples, the interleukin cytokine modulating potential, in both inhibiting the synthesis of cytokines and inhibiting the response caused by cytokine, of the various listed ingredients was measured and the results of each exposure was reported and evaluated.

For this evaluation, white blood cells were dosed with varying concentrations of the ingredients. An inflammatory stimulus, lipopolysaccharide (LPS), was then added to the ingredient-plus-cell culture, as well as untreated control samples of white blood cells. The production of the IL-1 cytokine of both the ingredient-treated cells and the untreated cells was compared to extrapolate the concentration of the ingredient that causes a 50% change in the concentration of IL-1 produced by the cells.

These examples are presented for purposes of further illustrating and explaining the present invention and are not to be taken as limiting in any regard. Unless otherwise indicated, all temperature measurements are in degree Celsius.

Example 1

To perform the evaluation of inhibiting interleukin synthesis of this example, human white blood cells, specifically THP-1 cells obtained from ATCC of Manassas, Va., were grown under conditions recommended by ATCC in a humidified 37° C. incubator, which was supplied with 5% carbon dioxide. To test the IL-1 inhibitory potential of various compounds, multiple groups of THP-1 cells were plated in 96 well plates. Specifically, 5×10⁵ THP-1 cells were plated in RPMI 1640 supplement with 10% fetal bovine serum and antibiotics, such as penicillin and streptomycin. With these cell samples plated, they were then dosed.

In dosing, each of the ingredients was administered to the plated THP-1 cells in varying doses, specifically, 100 ug/ml, 10 ug/ml, 1 ug/ml and 0 ug/ml. For example, in one plate of cells, 100 micrograms per milliliter of nettle root extract were administered, in another, 10 micrograms per milliliter were administered, in another 1 microgram per milliliter were administered, and in yet another no nettle root was administered. The THP-1 cells, now dosed with the desired ingredients, were returned to the incubator and incubated for 4 hours under the same conditions.

Thereafter, a known inflammatory stimulator, lipopolysaccharide (LPS) was added to each well plate to a final concentration of 100 nanograms/ml. The LPS treated cultures were returned to the incubator for an additional 16 hours. The cultures were then centrifuged at 1000 Gs for about 5 minutes to obtain a supernatant. The supernatants were then labeled and organized for IL-1 detection.

To detect secreted IL-1 in the different dosed supernatants and therefore evaluate the IL-1 secretion inhibitory potential of each dosage of each ingredient, each supernatant was tested for presence of IL-1 according to a conventional IL-1 testing protocol provided by the supplier of the tests, Biosource International of Camarillo, Calif. The specific protocol is provided in the reference, Protocol Booklet, Human IL-1, (2001), available from Biosource International, which is hereby incorporated by reference.

In general accordance with the protocol, 50 microliters of each supernatant sample was added to each well of anti-IL-1 coated plates, which were incubated at room temperature for 2 hours. Likewise, calibration standards designed to establish known amounts of IL-1 were added to the designated wells of anti-IL-1 antibody coated plates and incubated at room temperature for 2 hours.

Thereafter, the liquids from each of the wells were removed and the coated plate was washed 3 times in a wash buffer. After the third wash, 100 microliters of enzyme (horseradish peroxidase) conjugated anti-IL-1 antibody was added to each well of the coated plate and incubated at room temperature for 30 minutes. The plate was washed again as before and 100 microliters of substrate solution, (TMB), was added to each well to indicate amount of the IL-1 produced as a result of adding the LPS inflammation stimulator in presence or absence of the mentioned ingredients. Thereafter, 100 microliters of a stop solution, such as hydrochloric or sulfuric acid, was added to stop the reaction. The reaction of the substrate solution (TMB) and the horseradish peroxidase conjugated to the anti-IL-1 antibody produces a colored product with specific spectral absorbance at wavelength of 450 nm. Hence the amount of IL-1 present is determined by measuring the amount of absorbance at 450 nm resulting from the amount of bound enzyme conjugated anti-IL-1 antibody depending on the amount of IL-1 present.

The calibration standard samples were subjected to the same protocol as the supernatant samples to determine the IL-1 present in those calibration samples. The optical density of the calibration standard samples was tested to establish a known absorbance of IL-1 at varying levels, for example, 1500 to 50, 125, 62.5, 31.2 and 0 picograms per milliliter in calibration dilutent. The optical density of the standard calibration samples were then plotted to determine a standardized IL-1 absorbance calibration curve.

For each sample supernatant tested and optically measured, the absorbance was plotted against the standard IL-1 absorbance calibration curve to determine the amount of IL-1 present in the sample supernatant.

Based on the amount of IL-1 present in the sample supernatant, the results of the IL-1 inhibitory potential of the various ingredients was recorded as noted in Table III below. As indicated there, the results are reported in EC50 values, which is a commonly accepted measurement of effective dosages. Generally speaking, the EC50 is the concentration of ingredients required to cause 50% change in IL-1 concentrations when compared to control samples.

TABLE III Results of IL-1 Assays in Example 1 EC 50 in ug/ml (50% Ingredient Effective Dose in vitro) Epigallocatechin gallate <1 Grape seed extract <1 Grape seed extract <1 Ipriflavone <1 Nettle root extract <1 Rosehips <1 Tocotrienols 1-10 Borage oil 1-10 Evening primrose oil 1-10 Green coffee extract 1-10 INM-176 1-10 Boswellia serreta <1-10  Boswellia forte 1-10 Nettle root extract 1-10 Olive extract 1-10 Krill oil 1-10 Reishi mushroom 1-10 Afromomum melegueta extract 1-10 Feverfew 1-10 Artichoke 1-10 Ferulic acid 10

The results of the testing in Table III illustrate the effectiveness of these ingredients in reducing and/or inhibiting the secretion or production of cytokines, for example, IL-1.

Example 2

In this example, other ingredients that can be included in the composition of the invention were tested to (a) evaluate the effectiveness of these ingredients in reducing and/or inhibiting the secretion or production of cytokines, for example, IL-1; (b) evaluate interaction of the other ingredients with optional primary ingredients (rosehips, artichoke extract, olive extracts and nettle root); and (c) evaluate the effects of the other ingredients on interleukin cytokine, e.g., IL-1, response in hepatocytes by measuring the C reactive protein (CRP) secretion by hepatocytes.

Specifically, the release of IL-1 from the THP-1 cells after exposure to lipopolysaccharide (LPS) with or without pre-treatment in varying doses of the other ingredients, or a combination of those other ingredients with optional primary ingredients, was measured to evaluate the effects of the other ingredients on monocyte IL-1α secretion. Hepatocytes' response to IL-1 expression levels was evaluated by measuring CRP in the culture supernatant after exposure of the hepatocytes to IL-1 and IL-6 with or without pre-treatment with varying doses of the other ingredients.

The other ingredients tested were: blackberry extract, blueberry extract, cranberry extract, rosemary extract, clove extract and resveratrol. Table IV, demonstrates the results of these other ingredients for dose specific inhibition of IL-1α secretion upon activation via LPS in absence or presence of additional 4 primary ingredients at low dose. The methodology used to test the inhibition of IL-1α secretion is the same as that in Example 1 for the ingredients tested there. Of the ingredients tested in this Example 2, rosemary extract, blueberry extract, elderberry extract, cranberry extract had IL-1 modulatory effects at relatively low dose (ranging from 20-70 ug/ml).

Interference of the other ingredients with the IL-1 modulatory effects of the listed optional primary ingredients was tested by comparing the optional primary ingredients IL-1 secretion inhibitory effects, for example those shown in Table III, with the IL-1 secretion inhibitory effects of those primary ingredients in combination with the other ingredients listed in Table IV. For example, the presence of blackberry did not alter the IL-1 modulating effect of rosehips as illustrated by the absence of the characteristic inhibitory activity. None of the other ingredients showed interference with the optional primary ingredients, but some showed synergistic effects (blueberry extract showed synergistic effect with rosehips and nettle root, and elderberry extract with artichoke extract and nettle root). In Table IV, a “−” indicates no interference or no synergy, and a “+” indicates interference or synergy, respectively as shown.

The IL-1 response effects were evaluated in hepatocytes by measuring the secretion of CRP when the hepatocytes were exposed to IL-1α and IL-6 with or without pre-treatment with varying doses of the other ingredients. CRP secretion was chosen because, as noted above, CRP is a significant acute-phase protein in humans; its plasma concentration increases more than 1000-fold during severe trauma and/or inflammation; and CRP is expressed, e.g., synthesized or secreted, in hepatocytes in response to IL-1 and IL-6 under inflammatory conditions. CRP acts as a biomarker for interleukin cytokines, and it is efficient to measure this biomarker rather than the cytokines themselves because cytokine levels in the blood frequently do not accurately reflect the actual amount of the cytokines in the body. Thus, by measuring CRP secreted by the hepatocytes, the degree of cells' response to IL-1 and/or IL-6 is able to be determined.

To perform the evaluation of this example, human primarily isolated hepatocyte cells, obtained from In vitro Technology of Baltimore, Md., were restored under the conditions recommended by In vitro Technology in a humidified 37° C. incubator, which was supplied with 5% carbon dioxide. To test the CRP response potential of various compounds, multiple groups of hepatocytes were plated in 96 well plates. Specifically, 1×10⁴ cells were plated in hepatocyte media supplement with 10% fetal bovine serum which were then exposed with varying doses, specifically, 100 ug/ml, 10 ug/ml, 1 ug/ml and 0 ug/ml of test ingredients. For example: in one plate of cells, 100 micrograms per milliliter of blackberry extract were administered; in another, 10 micrograms per milliliter were administered; in another 1 microgram per milliliter were administered; and in yet another no nettle root was administered. With the plated cells treated with the respective ingredients, the hepatocytes, now dosed with the ingredient, were returned to the incubator and incubated for 4 hours under the same conditions.

Thereafter, a known CRP inducer, such as IL-1 and/or IL-6 was added to each well plate to a final concentration of 1 nanograms/ml. The IL treated cultures were returned to the incubator for an additional 16 hours. After the incubation, the supernatants were then labeled and organized for CRP detection.

To detect secreted CRP in the different dosed supernatants and therefore evaluate the CRP secretion inhibitory potential of each dosage of each ingredient, each supernatant was tested for presence of CRP according to a conventional CRP testing protocol provided by the supplier of the tests, Life Diagnostics, Inc. of West Chester, Pa. The specific protocol is provided in the reference, High Sensitivity C-Reactive Protein Enzyme Immunoassay Test Kit, (2003), available from Life Diagnostics, Inc., which is hereby incorporated by reference.

In general accordance with the protocol, 10 microliters of each supernatant sample was added to well of the anti-CRP antibody coated plates along with 100 ul of second antibodies against CRP conjugated to the horse radish peroxidase, which were incubated at room temperature for 45 minutes. Likewise, calibration standards designed to establish known amounts of CRP were added to the designated wells of anti-CRP antibody coated plates along with the second anti-CRP antibodies conjugated to the HRP and incubated at room temperature for 45 minutes.

Thereafter, the liquids from each of the wells were removed and the coated plate was washed 3 times in a wash buffer. After the third wash, 100 microliters of substrate solution, (TMB), was added to each wells to indicate amount of the CRP produced as a result of adding the IL-1 and IL-6, CRP inducer in presence or absence of the mentioned ingredients. Thereafter, 100 microliters of a stop solution, such as hydrochloric or sulfuric acid, was added to stop the reaction. The reaction of the substrate solution (TMB) and the horseradish peroxidase conjugated to the anti-CRP antibody produces a colored product with specific spectral absorbance at wavelength of 450 nm. Hence the amount of CRP present is determined by measuring the amount of absorbance at 450 nm resulting from the amount of bound enzyme conjugated anti-CRP antibody depending on the amount of CRP present.

The calibration standard samples were subjected to the same protocol as the supernatant samples to determine the CRP present in those calibration samples. The optical density of the calibration standard samples was tested to establish a known absorbance of CRP at varying levels, for example, 0, 0.005, 0.01, 0.025, 0.05, and 0.1 microgram per milliliter in calibration diluent. The optical density of the standard calibration samples were then plotted to determine a standardized CRP absorbance calibration curve.

For each sample supernatant tested and optically measured, the absorbance was plotted against the standard CRP absorbance calibration curve to determine the amount of CRP present in the sample supernatant.

Based on the amount of CRP present in the sample supernatant, the results of the CRP inhibitory potential of the various ingredients was recorded as noted in Table III below. As indicated there, the results are reported in EC50 values, which is a commonly accepted measurement of effective dosages. Generally speaking, the EC50 is the concentration of ingredients required to cause 50% change in CRP concentrations (e.g., a reduction in CRP or an inhibition of CRP synthesis) when compared to control samples

When IL-1 response effects were evaluated in hepatocytes in the form of CRP secretion, blackberry extract, cranberry extract and resveratrol had potent activity with EC50 of 1-10 ug/ml. Thus, these ingredients were effective at modulating IL-1/IL-6 response, specifically by reducing the synthesis of biomarkers, for example, CRP. Blueberry powder, rosemary extract and elderberry powder also had some activity in modulating IL-1/IL-6 responses. Additional, selected ingredients were tested for dose specific inhibition of CRP secretion upon activation via IL-1 and IL-6 (1 ng/ml each). Of the additional ingredients tested, 10% natural astaxanthin, Aframomum melegueta extract (w/o carrier) and Resveravine® (resveratrol) showed potent CRP lowering activity with EC50s of about 0.3, 0.6 and 0.8 ug/ml, respectively. Astaxanthin beadlet showed activity with EC50 of slightly over 1 ug/ml.

TABLE IV Example 2 Results Including EC50 of Other Ingredients In Inhibiting LPS Stimulated IL-1 Secretion, IL-1/IL-6 Stimulated CRP Secretion, and Synergistic or Antagonistic Effects With Optional Primary Ingredients CRP IL-1 EC EC 50 in 50 in ug/ml ug/ml (50% (50% Effec- Effec- tive tive Dose in Dose in Ingredient vitro) vitro) Synergy +/− Interference +/− Blackberry N/A  1-10 Rosehips − Rosehips − Powder Artichoke − Artichoke − Nettle Root − Nettle Root − Olive Extract − Olive Extract − Blueberry 30-40 10-20 Rosehips + Rosehips − Powder Artichoke − Artichoke − Nettle Root + Nettle Root − Olive Extract − Olive Extract − Cranberry >100  1-10 Rosehips − Rosehips − Extract Artichoke − Artichoke − Nettle Root − Nettle Root − Olive Extract − Olive Extract − Rosemary 20-30 30-40 Rosehips − Rosehips − Extract Artichoke − Artichoke − Nettle Root − Nettle Root − Olive Extract − Olive Extract − Clove Extract N/A N/A Rosehips − Rosehips − Artichoke − Artichoke − Nettle Root − Nettle Root − Olive Extract − Olive Extract − Resveratrol N/A  1-10 Rosehips − Rosehips − Artichoke − Artichoke − Nettle Root − Nettle Root − Olive Extract − Olive Extract − Elderberry 60-70 30-40 Rosehips − Rosehips − Extract Artichoke + Artichoke − Nettle Root + Nettle Root − Olive Extract − Olive Extract −

Example 3

In vivo testing will be conducted on approximately 150-200 human subjects by administering a composition of the invention in daily dosages over a twenty week period. More specifically, the composition will be tested to evaluate (a) the inhibition of IL-1 synthesis in Pattern 1 individuals, and (b) evaluate the inhibition of IL-1 response as measured by CRP levels in Pattern 2 individuals having CRP levels above 3 mg CRP/L blood.

For purposes of this example, Pattern 1 individuals are humans who have of IL-1 genotypes known to possess “normal” or “exaggerated” IL-1 gene expression responses. The exaggerated IL-1 gene expression genotype is defined as the presence of at least one copy of allele 2 at the +3954 locus of the IL-1α gene or two copies of allele 2 at the +4845 locus of the IL-1α gene. In this example, Pattern 2 individuals are humans who have elevated blood levels of inflammation biomarkers, for example, CRP levels of about 3 to about 10 mg/L or more. With this range of CRP, there appears to be a greater opportunity for observing noticeable improvement, i.e., reduction of CRP levels. In other words, it likely will be possible to detect modest decreases in CRP in Pattern 2 subjects.

One composition of the invention that will be administered to fifty test subjects in tablet form daily will include rosehips, blackberry and blueberry, in the daily amounts: 1200 mg rosehips, 165 mg blackberry and 330 mg blueberry.

Another composition of the invention that will be administered to fifty test subjects will include rosehips, blackberry, blueberry and Aframomum melegueta, in the daily amounts: 1200 mg rosehips, 165 mg blackberry, 330 mg blueberry and 300 mg Aframomum melegueta. These ingredients will be in tablet form.

A further composition of the invention that would be administered to 50 test subjects will include rosehips, Aframomum melegueta and resveratrol (e.g., Resveravine®, a resveratrol product commercially available from Bio Serae of Bram, France) in the daily amounts 1200 mg rosehips, 150 mg Aframomum melegueta and 40 mg resveratrol. These ingredients will be in tablet form.

A placebo, including no ingredients of the composition, will be administered daily in tablet form to fifty subjects.

The first twelve weeks of administering the above compositions and placebo to the respective subjects will be the double blind phase of the testing. During this phase the dosages above will be the loading dose. After the twelfth week, the single blind phase of testing will begin. During this phase, the dosages above may be altered to maintenance dosages, which will be extrapolated from the loading phase data.

In the in vivo testing of this example, blood samples will be taken from the subjects to measure IL-1 and CRP. This blood sampling will be controlled, however, to quantify and minimize intra-individual variability. For example, inflammation biomarkers, such as CRP, and gene expression outcomes, such as the transcription and subsequent synthesis of IL-1, will be measured in multiple, fasting blood samples; and samples from women will be obtained during the same week as those subjects' monthly menstrual cycle. The reason for this is that it has been discovered that high fat meals and menstrual cycles acutely increases inflammation biomarkers. Further, this CRP monitoring protocol suggests taking multiple sampling on different, proximate days.

After administration, the efficacy of the placebo, first and second compositions will be evaluated by ex vivo IL-1 production inhibition (see methods above), in vivo IL-1 gene expression, and blood levels of inflammatory biomarkers, e.g., IL-6, TNF, IL-10, IL-12, Serum Amyloid A, Fibrinogen, ICAM-1, C reactive protein, etc. In vivo IL-1 gene expression will be evaluated by measuring the amount of IL-1 mRNA in subject leukocytes by rtPCR. Evidence of an effective intervention for regulation of the inflammatory process include inhibition of ex vivo IL-1 production, inhibition of in vivo IL-1 gene expression, and decreased levels of one or more inflammatory biomarkers as listed above.

The results of the in vivo testing of this example are expected to illustrate that the composition has the effect of modulating interleukin cytokines by both inhibiting the synthesis of interleukin cytokines and inhibiting the cascading response caused by interleukin cytokines in the subjects. A particular example of this benefit will now be described in connection with a subject having atherosclerotic plaque development exacerbated by inflammation, with reference to FIGS. 1-3.

FIG. 1 illustrates how inflammation and interleukin cytokines contribute to atherosclerotic plaque development. Specifically, mononuclear leukocytes 10 enter artery wall tissue 100, engulf oxidized lipids, recruit additional leukocytes 14 with inflammatory cytokines, e.g., IL-1, stimulate plaque growth 30 via growth factors, which results in stenosis. The cytokines 20 released by the leukocytes within the plaque lead to plaque instability, and can ultimately cause rupture and thrombosis.

In FIG. 2, the synthesis of IL-1 200 and the IL-1 response 300 is shown. Specifically, the leukocytes 10 synthesize the cytokines 20, which in turn stimulate liver 50 production of acute phase proteins, e.g., CRP.

With reference to FIG. 3, the composition of the invention inhibits the production of the cytokines 200 by the leukocytes 10. Specific ingredients 500, e.g., rosehips, blueberry and elderberry, can be targeted to perform this function. The composition of the invention may also inhibit the response 300 of cells and/or the liver 50 to the cytokines by reducing or eliminating the synthesis of CRP. Specific ingredients 600, e.g., blackberry, resveratrol, Aframomum melegueta and/or astaxanthin can be targeted to perform this function.

Example 4

The in vivo effects of daily administration of three different compositions of the present invention were periodically evaluated in 120 human subjects over twelve weeks using a double blind, placebo-controlled trial. In particular, the effects of the compositions of the present invention on recognized markers of inflammation, such as plasma levels of C-reactive protein (CRP), as well as other markers of inflammation, such as ex vivo IL-1 production and in vivo IL-1 synthesis and/or expression, were evaluated. All human subjects participating in this trial were at least 18 years of age, judged to be in good general health on the basis of an interview and abbreviated physical exam, willing to maintain their normal dietary and exercise habits throughout the trial, a non-smoker for at least a year, and had an average CRP level between 2 mg/L and 10 mg/L.

All human subjects analyzed in this trial fell into one of two groups, (1) subjects having IL-1 Pattern 1 genotype and (2) subjects having IL-1 non-Pattern 1 genotypes. As described above, a human subject with an IL-1 Pattern 1 genotype is generally known to possess “exaggerated” IL-1 gene expression responses. IL-1 Pattern 1 genotype is defined as the presence of at least one copy of allele 2 at the +3954 locus of the IL-1 gene [IL-1α (4845)=1.2 and IL-1α (+3954)=1.2 or 2.2] or two copies of allele 2 at the +4845 locus of the IL-1α gene [IL-1α (+4845)=2.2]. A subject having an IL-1 non-Pattern 1 genotype may have any genotype other than Pattern 1 as defined herein.

All subjects were randomly assigned to one of four groups. Group 1 comprised 10 subjects of Pattern 1 genotype and 22 subjects of non-Pattern 1 genotype. Group 1 was administered composition 1, as described below in Table V. Group 2 comprised 9 subjects of Pattern 1 genotype and 22 subjects of non-Pattern 1 genotype. Group 2 was administered composition 2, as described below in Table V. Group 3 comprised 10 subjects of Pattern 1 genotype and 20 subjects of non-Pattern 1 genotype. Group 3 was administered composition 3, as described below in Table V. Group 4 comprised 12 subjects of Pattern 1 genotype and 15 subjects of non-Pattern 1 genotype. Group 4 was administered a placebo.

Table V lists the ingredients of each composition of the present invention tested in this trial, as well as the dosage regimen for each.

TABLE V Compositions of Tablets and Dosage Regimens for Tablets Administered According to Example 4 Daily Dose (to be taken all at Composition of tablet to be administered: once in the a.m.) Composition 1: 300 mg rosehips powdered extract + 4 active tablets 41 mg of blueberry powdered extract + 83 mg of blackberry powdered extract + 75 mg of Aframomum melegueta extract per tablet Composition 2: 300 mg rosehips powdered extract + 4 active tablets 41 mg of blueberry powdered extract + 83 mg of blackberry powdered extract + 10 mg resveratrol (e.g., Resveravine ®, a resveratrol product commercially available from Bio Serae of Bram, France) per tablet Composition 3: 300 mg rosehips powdered extract + 4 active tablets 10 mg resveratrol (e.g., Resveravine ®, a resveratrol product commercially available from Bio Serae of Bram, France) + 75 mg Aframomum melegueta extract per tablet Placebo 4 placebo tablets

Regardless of group assignment, subjects were instructed to consume the same number of tablets once daily, as illustrated by Table V. All tablets comprising a composition of the present invention (Compositions 1-3), and all placebo tablets were identical in appearance. Inert excipients such as microcrystalline cellulose, corn starch, dicalcium phosphate and processing aids such as modified cellulose gum, magnesium stearate and silicon dioxide were added to the tablets comprising the compositions of the present invention to allow processing of the compositions into tablets.

Placebo tablets matched the tablets of Compositions 1, 2, and 3 in size and appearance. The placebo tablets contained a combination of inert excipients. For example, the placebo tablets may have contained one or more of microcrystalline cellulose, corn starch, dicalcium phosphate, and processing aids such as modified cellulose gum, magnesium stearate and silicon dioxide.

During the course of the trial, each subject underwent eleven periodic (approximately weekly) analyses as outlined below. Each analysis included an abbreviated physical exam of the test subject that included measurement of vital signs, body weight, assessment of concomitant medical use, assessment of compliance with taking the administered tablets for the trial, and screening for adverse experiences. Additionally, during approximately every other periodic analysis (approximately every 2 weeks), serum levels of various molecules including CRP, glucose, creatinine, uric acid, SGOT, SGPT, total bilirubin, albumin, sodium chloride, BUN, calcium, LDH, alkaline phosphatase, total protein, potassium, CO₂, GGT, phosphorus, magnesium, and CPK were analyzed. Any subject who showed abnormal serum levels was re-tested approximately a week later. Further, a panel of cytokine inflammatory biomarkers, including IL-1β, TNF-α, IL-6, IL-8, IL-10, and IL-12 was analyzed for each subject on approximately a bi-weekly basis. Even further, during approximately the seventh and eleventh weeks, blood and urine samples were taken from each subject and hemoglobin, hematocrit, and a blood count were measured for each blood sample, while the urine sample was analyzed for color, specific gravity, pH, protein, glucose, ketones, bilirubin, nitrite, leukocyte esterase, urobilinogen, white blood cells, epithelial cells, and bacteria.

In this example, blood sampling was controlled to quantify and minimize intra-individual variability. For example, as outlined above, inflammation biomarkers, such as CRP, and gene expression outcomes, such as the transcription and subsequent synthesis of IL-1, were measured in multiple, fasting blood samples. Blood samples from female subjects were obtained during the same week as those subjects' monthly menstrual cycle. This method of blood sampling is designed to control acutely increased expression of inflammation biomarkers, which often occurs in response to high fat meals and during menstrual cycles.

Periodically, e.g. at four weeks, eight weeks, and twelve weeks after initiation of administration, efficacies of the placebo, Composition 1, Composition 2, and Composition 3 were evaluated by (a) ex vivo analysis of inhibition of IL-1 synthesis and/or expression in the test subjects (see methods above); (b) analysis of in vivo IL-1 gene expression (rtPCR was used to measure IL-1 mRNA levels in samples obtained from test subjects); and (c) analysis of serum and/or cellular synthesis and expression levels of inflammatory biomarkers, such as IL-6, TNF, IL-10, IL-12, Serum Amyloid A, Fibrinogen, ICAM-1, C reactive protein, etc.

The results of these evaluations are reported FIGS. 4-15. The graphs shown in FIGS. 4-15 are normalized to baseline values, and are expressed as a fraction or fold of baseline value (percent of baseline divided by 100).

FIG. 5 shows that after twelve weeks of administration of Compositions 1, 2, and 3, in the total study population there was a decrease in CRP synthesis and/or expression compared with administration of a placebo.

As shown in FIG. 7, when CRP response in Pattern 1 subjects was analyzed separately from response in non-Pattern 1 test subjects, there was a pronounced difference between all active treatments versus baseline and placebo. In particular, Composition 2 (rosehips+berries+Resveravine® ((Resveravine® is a resveratrol product commercially available from Bio Serae of Bram, France) (P=0.0113)) inhibited CRP synthesis and/or expression at a rate of approximately 50%.

FIG. 8 illustrates that ex vivo IL-1 synthesis was slightly decreased compared to placebo and baseline levels following twelve weeks of administration of Compositions 1, 2, and 3. No significant difference was observed between Compositions 1, 2, and 3.

FIG. 10 illustrates that in the test subjects having a Pattern 1 genotype, there was a striking divergence of response between compositions of the present invention and placebo compositions. In particular, after twelve weeks, IL-1 production was reduced by approximately 20%-30% in each of Groups 1, 2, and 3 relative to baseline (P=0.0068).

As shown in FIG. 12, analysis of the baseline versus 12 week data in the total subject population reveals that IL-1 gene expression was significantly reduced following administration of each of Compositions 1, 2, and 3, with the most striking result associated with administration of Composition 2 (P=0.0047).

As FIG. 14 illustrates, when data for test subjects having a Pattern 1 genotype were evaluated independently, IL-1 gene expression declined by approximately 80% relative to baseline. This decline was highly significant in Group 2, which was administered Composition 2 (Composition 2 comprises Rosehips, Berries, and Resveravine® (Resveravinee is a resveratrol product commercially available from Bio Serae of Bram, France) (P=0.0158)).

FIGS. 4, 6, 9, 11, 13, and 15 illustrate that the test subjects who were administered a placebo experienced a sharp increase in CRP expression levels, IL-1 ex vivo expression levels, and IL-1 gene expression levels after four weeks of administration. Without being limited to any theory, it is believed that this sharp increase may have been due to a change in the dietary habits of the test subjects. The change in dietary habits may be due to the concurrence of certain holidays and the clinical trial.

Significantly, each of FIGS. 4, 6, 9, 11, 13, and 15 also illustrate that the test subjects who were administered any of Compositions 1, 2, or 3 did not exhibit the sharp increase in CRP expression levels, ex vivo expression levels of IL-1, or IL-1 gene expression levels at point during the clinical trial. Rather, these individuals maintained a steady or decreased state of expression of CRP and IL-1. Thus, even though Compositions 1, 2, and 3 did not always reduce CRP expression levels, ex vivo expression levels of IL-1, or IL-1 gene expression levels, each of Compositions 1, 2, and 3 did modulate the immune response by preventing a sharp increase in expression levels of CRP and IL-1. One example of this modulatory effect may be seen in FIGS. 4, 6, 9, 11, 13, and 15 at the 4 week marker.

The results shown in FIGS. 4-15 demonstrate that Composition 2, which comprises rosehips+berries+Resveravine® (Resveravine® is a resveratrol product commercially available from Bio Serae of Bram, France), selectively down regulates IL-1 gene activity, including synthesis and expression, in subjects having a Pattern 1 genotype. The magnitude and temporal pattern of IL-1 gene expression, IL-1 production, and CRP expression and/or synthesis observed in this example are consistent with the current paradigm wherein exaggerated IL-1 gene responses account for an augmented inflammatory environment in subjects having a Pattern 1 genotype.

In striking contrast to the results in Pattern 1 subjects, the anti-inflammatory effects observed for non-Pattern 1 subjects were much less clear. This genotype (IL-1 haplotype) differential response suggests that Composition 2 (i.e., rosehips+berries+Resveravine® (Resveravine® is a resveratrol product commercially available from Bio Serae of Bram, France)), selectively down regulates the inflammation pathway at the level of IL-1 gene activity in Pattern 1 individuals, leading to clinically meaningful decreases in established downstream markers of inflammation, e.g., CRP.

V. Methods of Use

Based on the results of the testing of ingredients that can be included in compositions according to the present invention as exemplified by Examples 1-4 above, it is evident that compositions including these ingredients can be administered to subjects to modulate inflammatory responses by modulating mediators of inflammation, such as interleukin cytokines. More specifically, it is evident that the compositions of the present invention can be used to specifically inhibit synthesis of an interleukin cytokine, such as interleukin-1 (IL-1), for example, by inhibiting mRNA transcription of IL-1. Further, as evidenced by the results reported herein, the compositions of the present invention can be used to specifically inhibit the expression/secretion of interleukin cytokines such as IL-1. Even further, it is evident that the compositions of the present invention can modulate an inflammatory response by decreasing synthesis and/or expression/secretion of a biomarker of inflammation such as CRP. A decrease in synthesis and/or expression/secretion of CRP inhibits the response of CRP to interleukin cytokines in the body of the subject. Still further, it also is evident that the compositions of the present invention can modulate the immune response by preventing increases in expression, synthesis, and/or secretion levels of mediators of inflammation, such as CRP and IL-1.

The results described herein demonstrate that daily administration of three different compositions of the present invention achieved a reduction in IL-synthesis and expression, as well as a reduction in CRP synthesis and/or expression/secretion in the overall subject population. However, the most striking evidence of the ability to modulate an inflammatory response utilizing compositions of the present invention occurred in the subject population having a Pattern 1 genotype. For example, daily administration of Composition 2 (e.g. Rosehips, berries and Resveravine® (Resveravine® is a resveratrol product commercially available from Bio Serae of Bram, France) in the disclosed dosage amounts) achieved a reduction in IL-1 gene expression of approximately 80% (relative to baseline) and a reduction of approximately 50% in the expression of the inflammatory biomarker CRP after twelve weeks in the subject population having a Pattern 1 genotype.

These results are significant because inflammation is a major component of numerous diseases including cardiovascular diseases. Indeed, given that inflammatory cytokines, such as IL-1 and IL-6, are key mediators of the inflammatory response in the body, it is significant that the compositions of the invention can be used to modulate the inflammatory response associated with diseases and/or abnormal conditions. For example, the compositions of the present invention may be used to modulate the inflammatory response in subjects suffering from arthritis, cardiovascular conditions, osteoporosis, Alzheimer's disease, an inflammatory response to a traumatic injury, or any other abnormal conditions or diseases associated with inflammation or immunomodulatory responses, such as pain and stiffness associated with inflammation.

In a further example, osteoporosis has been linked to abnormally high IL-1 release levels. Therefore, contemplated within this invention is a method for treating osteoporosis by modulating IL-1 synthesis and expression through administration of a therapeutically effective amount of the composition of the invention.

Given the connection between inflammation and cardiovascular diseases, such as atherosclerosis, thrombosis, aortic stenosis, etc. also contemplated within this invention is the treatment of such cardiovascular diseases by administering a therapeutically effective amount of the composition including at least one of the ingredients in a recited dosage. Indeed, inflammation in cardiovascular diseases is associated with increased synthesis and expression levels of IL-1. Expression of IL-1 triggers synthesis, expression and secretion of CRP, which is believed to stimulate further IL-1 synthesis and/or expression. Therefore, a composition of the present invention, such as Composition 2 which is shown to reduce levels of CRP by approximately 50% and reduce levels of IL-1 gene activity by approximately 80%, is useful in modulating an inflammatory response and thereby treating cardiovascular conditions.

Additionally, it is believed that the IL-1 regulating composition of the invention may be used to limit or to control an inflammatory response due to surgery, trauma and allergic reactions involving the skin, lungs, eyes, ears, nose, throat, digestive tract, nervous system and the like.

Furthermore, it has been observed that nervous system cells and tissue secrete significant amounts of IL-1 and when stressed under inflammation, and therefore, the composition may be used to treat mental problems, headaches and earaches, as well as debilitating neurological diseases such as Alzheimer's diseases.

The above descriptions are those of the preferred embodiments of the invention. Various alterations and changes can be made without departing from the spirit and broader aspects of the invention as defined in the appended claims, which are to be interpreted in accordance with the principles of patent law including the doctrine of equivalents. Any references to claim elements in the singular, for example, using the articles “a,” “an,” “the,” or “said,” is not to be construed as limiting the element to the singular. 

1. A dietary supplement for modulating an immune response in a subject comprising rosehips, at least one of resveratrol and viniferin, and a blueberry ingredient, to provide a daily dosage of about 300-1500 mg rosehips, about 41-500 mg blueberry ingredient, and about 10-150 mg resveratrol or viniferin wherein the dietary supplement is effective for reducing the expression of an inflammatory cytokine.
 2. The dietary supplement of claim 1, wherein the inflammatory cytokine is interleukin-1.
 3. The dietary supplement of claim 1, further comprising a blackberry ingredient.
 4. The dietary supplement of claim 1, further comprising at least one of Aframomum melegueta extract and a blackberry ingredient.
 5. The dietary supplement of claim 1, wherein the subject possesses an interleukin-1 (IL-1) pattern 1 genotype.
 6. The dietary supplement of claim 1, wherein a single dosage unit of the dietary supplement comprises about 300-1500 mg of rosehips, about 41-500 mg of blueberry ingredient, and about 10-150 mg of resveratrol or viniferin.
 7. The dietary supplement of claim 4, wherein the dietary supplement further provides a daily dosage of about 50-300 mg of blackberry ingredient and about 75-500 mg of Aframomum melegueta extract. 